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For both adhesion tests, samples were allowed to cure overnight prior to testing.
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Test samples were allowed to react with stable free radical, 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH, Wako Chemicals USA, Inc).) for half an hour at 37°C.
The test sample was allowed to react simultaneously with the antibodies, resulting in the ferritin molecules being sandwiched between the solid phase and enzyme-linked antibodies.
We also challenged this CAM test to estimate the origins of LWC chimpanzees originally placed in southern Cameroon by the Cameroon-Nigeria bounded CAM tests; except in this test these LWC samples were allowed to originate anywhere within the range of P. t. troglodytes.
Samples were allowed to acclimatize to ambient temperature before testing.
These bone samples were allowed to thaw at room temperature before biomechanically tested.
The samples were allowed to dwell on the skin for 10 min prior to testing.
Following the forced swim test animals were allowed 1 week to recover before samples were collected for gene expression profiling.
After each drop, the test sample shall be allowed to come to rest and shall be examined and evaluated before continuing.
Blind test samples were prepared by Mary Eubanks.
They were allowed to compete at Barcelona because the "B" samples of their urine specimens had not yet been tested.
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