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Other statistics include tests of population differentiation (Fst, reflecting the level of genetic isolation between populations).
Exact tests of population differentiation were also performed among all pairs of populations using Genepop [42], [43].
Tests of population stratification, batch effects, SNPs disrupting probe hybridization, and systematically inflated p-values all showed the lack of any confounding factors (see Materials and Methods).
Here, pairwise tests of population differentiation were significant between M. faveolata and M. annularis on North Norman's Patch Reef (P<0.005); however, similar comparisons between South Perry M. faveolata and M. annularis were not significant (P>0.005).
Most exact tests of population differentiation were significant but some populations, in Central (SLO1, HU2, HU6, HU3) and Eastern Europe (PO2, UKR1, UKR2, RU4 and RU5), appeared clearly less differentiated from North American populations than the others (Table S2).
Formal tests of population stratification using genomic control were not conducted.
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Summary statistics and neutrality tests of populations at species boundaries.
The results obtained from the exact test of population differentiation, for the 15 STR loci, also show wide diversity among the Adi sub-tribes.
In addition, the test of population homogeneity confirmed that our population was homogeneous, as the clusterization of individuals was not significant (lambda = 1.0032 for ΔDBP120, lambda = 1.0044 for ΔSBP120 and lambda = 1.0008 for PNat120).
Locus-wise exact test of population differentiation, using Arlequin 3.01, was performed to analyze the extent of genetic diversity at the studied loci among the six Adi sub-tribes [75].
Tajima's D [ 110] analysis was performed with ARLEQUIN as an additional test of population expansion.
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