An ultimate validation of the entire reconstruction was attained by the experimental testing of growth phenotypes (an ability of a given strain to grow on a specific sugar substrate as a sole source of carbon and energy) predicted by the presence or absence of respective reconstructed pathways.
We quantitatively compared these strains by performing paired t tests of their growth rates (extracted from their growth curves) (Table S1).
NTM were further identified by following conventional tests: rate of growth, pigment production, arylsulfatase, heat-stable catalase, growth on MacConkey agar without crystal violet, iron uptake, nitrate reduction, tween-80 hydrolysis, tellurite reduction and urease production [ 13].
Inhibition tests of batch growth were performed according to a full factorial design with three factors (ferrous iron, limestone and olive pomace) taken at two levels.
The demographic history of populations was inferred using tests of population growth.
This was reflected in the hierarchical cluster analysis where there was an overall high degree of correlation (> 90%) in gene expression in each EOC cell line group tested irrespective of growth condition.
Student's t-test was used to test the effect of growth medium on OD600 (cell density).
Hormonal tests demonstrated elevation of growth hormone and somatomedin C. The patient was diagnosed with stabbing headache, secondary to acromegaly.
Although two different media formulations were used for the MIC tests, the extent of growth for all three bacterial strains was comparable.
