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For pox antigen we used a live virus vaccine for Fowl Pox Virus (FP-VAC; Intervet/Schering-Plough), following tests of binding by Darwin's finch antibodies (see below) and based on the likely occurrence of conserved antigens among Fowl Pox and Canary Pox [25].
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Expression and testing of binding of antibodies in intermediate nodes of these phylogenies could be used to experimentally validate the relevance of the inferences.
Compatibility testing of bindings reduces the scope for errors arising due to the binding of incompatible interfaces, in turn reducing overhead for the application composer.
To distinguish between these two scenarios, we used chromatin immunoprecipitation (ChIP) to test for binding of the protein to upstream regulatory regions of the putative targets.
Aliquots of the fractions that were coloured by anisaldehyde on thin-layer plates were tested for binding of the CS6 protein using the chromatogram binding assay.
As loading controls, the same nuclear extracts were tested for binding of Oct-1 (octamer-binding protein-1) to an Oct-1 consensus probe (5'-TGTCGAATGCAAATCACTAGAA-3').
Importantly, alveolar macrophages and BMDmØ expressed highly SR-B1, therefore these cell populations were tested for binding of BCG-lux (Fig. 3B).
As a preliminary test of whether binding of ATF3 and/or JUN to these AP-1 sites might be involved in their expression in EMT6 cells, we performed ChIP experiments with antibodies against either ATF3 or JUN.
Homozygous mutants allowed the testing of IgE binding on the altered proteins from seed extracts.
The experiment was based on testing of antibody binding to HER2 proteins that have been modified by deletion of different subdomains of its ECD.
Each internal node corresponds to a test of the binding of a selected TF to a gene, for example, "can TF A bind to gene g?".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com