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Chi-square tests for segregation distortion were performed for each EST-SSR marker using log-likelihood ratio statistics (G) of G-MENDEL 3.0 [ 47].
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The remaining family specific variants then have to be tested for segregation with disease in the pedigree.
After filtering, the remaining variants may be reduced to a few family-specific variants, which are thereafter tested for segregation within the whole family.
The resulting SSR genotyping data were tested for segregation ratio of 1 2 1 in the F2 populations using a χ2 goodness-of-fit test, at a significance level of P < 0.005.
We used amplified fragment length polymorphisms (AFLPs) to test for segregation distortion in one hybrid cross between green and longear sunfish (Lepomis cyanellus and L. megalotis).
Testing for segregation in the families of the probands carrying the A438T and G787R variants was performed by direct sequencing, using either lymphocyte or archival DNA from paraffin-embedded tissue blocks.
We tested for segregation distortion of amplified fragment length polymorphisms (AFLPs) [16] in one partially viable interspecific cross between the green and longear sunfishes, Lepomis cyanellus and L. megalotis [17].
Markers were tested for segregation distortion by the chi-square test.
Sequence changes were tested for segregation with the condition in participating relatives.
SNP markers were initially tested for segregation distortion using the chisq module of TMAP [ 38].
These markers were scored in the 92 progeny and tested for segregation distortion.
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