The RNA samples were tested for absence of inhibitors using the SPUD assay - no inhibitors were detected [36].
The predictive value of a negative HPV test for absence of pathology amongst women with no cytological abnormalities has recently been reported in a more controlled study (ATHENA) as over 95%.
All flake food is tested for absence of estrogenicity using the yeast estrogen screen assay (Routledge and Sumpter, 1996) prior to use.
In addition, calibration data were tested for absence of heteroscedasticity, since this would change the uncertainty of analytical results in a concentration dependent manner.
All cell lines have been tested for absence of mycoplasma contamination with MycoAlert (Lonza) -last control March 2013 -and authenticated by STR analysis using genRESVR MPX-2 and genRESVR MPX-3 kits (serac, Bad Homburg, Germany).
Even in the best case scenario (prevalence of 0%), the likelihood ratio of a negative test for absence of malaria was 5, and it was less than 5 in all other situations (detailed results not shown).
With intercept considered an artifact in this particular case, linear regression provided better fitting and is therefore considered the correct approach Additionally, calibration data were tested for absence of heteroscedasticity, since this would entail the uncertainty of analytical results to vary in a concentration-dependent manner.
As in note 3, the influence of market intermediaries on vertical price transmission could not be tested empirically for absence of adequate data.
We extended our analysis to include the full set of 22 pluripotency transcription factors regardless of orthology, by testing solely for absence or presence of signal peaks identified by MACS (i.e., without considering enrichment scores; see 'Materials and methods').
To verify the purity of the isolated GCTSCs they were tested for the absence of the monocytic/histiocytic markers CD163 and CD34 and the absence of colony stimulating factor 1 receptor (CSF1R) expressed by giant cells by RT-qPCR as described earlier [ 8].
