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The most appropriate model of nucleotide sequence evolution was identified using likelihood ratio tests as implemented in Model Test [ 48].
Mantel tests as implemented in PRIMER v6 were also assessed to analyze the relationships between phylogenetic distances of bacterial communities and soil properties.
Relationships between phylogenetic distances of bacterial communities and soil properties were assessed by Mantel tests as implemented in PRIMER v6 (Clarke and Gorley [2006]).
Another method of model validation is the use of various statistical tests, as implemented in the ENMtools software [88], [90].
Fisher's exact tests on 2×2 contingency tables were used for McDonald-Kreitman tests, as implemented in DnaSP [66].
Significant differences in the likelihood among all trees were tested by the AU, Shimodaira-Hasegawa (SH), and Kishino-Hasegawa (KH) tests as implemented in Consel 0.1i [91], [91].
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A Mantel test as implemented in Mantel Nonparametric Test Calculator, version 2.0 [ 110] was used to test for isolation by distance within identified lineages/haplogroups.
Wilcoxon test as implemented in the R programming language was utilized, part of the "stats" package, with confidence interval 99% and alternative hypothesis being "greater" [74].
Transcripts that expressed significant differences between the groups of the most sensitive and most resistant cell lines were determined using moderated F-tests as implemented in the Bioconductor package limma [24].
Haplotype blocks were identified using the 4 gamete test as implemented in Haploview.
The 'best close match' test as implemented in TaxonDNA yielded comparable results (Fig. 1b, Table 2).
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