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In the spring of 2012, two years after the first round of tests, a subset of the same carpenter apprentices – those who were still doing their apprenticeship – were administered the same test a second time.
In most tests, the set of reference frequencies was calculated using all octamers, as described by Tsirigos and Rigoutsos, but in some tests a subset of octamers were used, determined as described above.
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To identify these items, it is possible to perform group tests: testing a subset of the population will lead to a positive test result if this subset contains at least one defective item, and a negative result otherwise.
We used qPCR to test a subset of these transcripts and found that 83% were confirmed in the same samples, and 86% of these were again confirmed in an independent sample.
Finally, we tested a subset of these FRET pairs in the Epac sensor.
We further tested a subset of proteins found by mass spectrometry to be upregulated in plasma from tumor bearing mice, for their levels in human and murine plasma.
To rule out other blood-borne infections, we further tested a subset of 30 lions for infection with Mycoplasma hemofelis and M. hemominutium, only finding M. hemominutium.
In order to indentify miRNAs that potentially drive the proliferation process in breast cancer we tested a subset of the HP vs. LP differentially miRNAs using high-throughput lysate microarray (LMA) screening technology [28].
We tested a subset of the CNEs, each associated with unique sequence features, as potential enhancers in transient transfection assays in H661 cells to see if the core elements conserved in human-Fugu represent functionally critical regulatory modules.
We tested a subset of Hox and Hox cofactor genes, HOXA5, HOXA7, HOXA9, HOXA10, PBX1, PBX2, PBX3, and MEIS1, and possible Hox target genes FLT3, HLF, JAG2, MEF2C, DDX4, and PRNP.
For reliability testing a subset of subjects were included.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com