This test was started with an incline of +5° and a speed of 6 m/min for 5 min. After this initial phase, the speed was progressively increased by 2 m/min every 3 min. Animals ran until exhaustion, which was defined as the inability to remain on the treadmill despite repeated contact with the electric grid (0.4 mA) and mechanical prodding for more than 10 s.
However, the rate was clearly faster when the test was started with inoculum from the digesters with TE addition (Table 3).
After careful calibration, the hemodynamic parameters at rest were measured again and the exercise test was started with a workload of 25 Watts.
The backward selection procedure using the Cochrane Armitage trend test was started with the 11 mutations (10, 33, 36, 46, 54, 62, 71, 73, 82, 84, 90) selected with the most restrictive criteria (p < 0.01) to avoid computational issues.
All tests were started with an inoculum of 5000 cells ml−1 obtained from a preculture in its exponential growth phase.
All tests were started with offspring (less than 24-h-old, at least second brood offspring) obtained from stock cultures kept at environmental conditions similar to the tests.
To avoid excessive stimulation, the testing was started in the following sessions with the weakest hair that had elicited withdrawal responses in the previous session.
Before testing was started, each mouse received three training sessions with water in the gustometer.
For the next part of these tests the game was started with a client connected, to place their required objects within the building.
Accordingly, aside from the standard OD600 0.6 for induction we also tested expression when induction was started with culture at OD600 1.0.
