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Blood for serologic testing was collected in standard marble-top vacutainer™ tubes and stored refrigerated for not more than 48 hours before being centrifuged.
Each blood sample (that is, two baseline samples, one sample immediately after pain testing, then samples at 15, 30, and 60 minutes following the conclusion of pain testing) was collected in a 10-mL tube and transported to the GCRC Core Laboratory, where it was centrifuged, aliquoted, and stored in a -80°C freezer for later assay.
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Spot urine samples for further biochemical testing were collected in the morning on the day of the clinical appointment.
Urine samples for nicotine and cotinine testing were collected in urine cups without preservative and refrigerated until shipping to a clinical lab (Diagnostic Laboratory of Oklahoma, Oklahoma City, OK).
Samples for plasma glucose testing were collected in fluoride/oxalate tubes and then centrifuged and assayed onsite using a YSI 2300 STAT PLUS instrument.
Samples for HbA1c testing were collected in EDTA tubes and processed at Victoria Hospital in Quatre Bornes using high-performance liquid chromatography on a Tosoh G7 automated system (Tosoh, Tokyo, Japan).
Blood for the renal function tests was collected in sterile empty vials.
The results of the tests are collected in Table 1.
Samples for batch test were collected in June and September.
In Section 3 the ELH method and its implementation are described, while the results of the numerical tests are collected in Section 4.
The clinical, laboratory, and radiographic data and the results of QFT test were collected in both groups.
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