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Titers were determined by testing twofold serial dilutions of mice sera.
The virus neutralization (VN) assay was carried out in 96-well plates, testing twofold dilutions of sera against a fixed dose of vaccinia Araçatuba virus (100 TCID50 [50 % tissue culture infectious dose]).
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In the quantitative test, twofold serial dilutions (from 1 2 to 1 16,384) were prepared using normal non-reactive human plasma converted to serum 8 as a diluent.
In each test run, a positive control serum with known titer was tested in twofold serial dilutions to guard the sensitivity, and results were crosschecked by two experienced technicians.
Context effects in recognition tests are twofold.
The main goal of the GEYSERS testbed is twofold.
The authors tested a twofold hypothesis: a self-etch, or SE, adhesive would result in less postoperative sensitivity than a total-etch, or TE, adhesive; an SE adhesive would result in poorer enamel marginal integrity than a TE adhesive.
The goal of this test was twofold.
Twenty-five microfiters of DMEM were added to each well and, for each serum tested, serial twofold dilutions were made.
The antibacterial activity of CFS against S. Typhimurium was tested at twofold serial dilutions using agar-WDA as described above.
To study this issue, CSF and serum samples were tested in twofold dilution series with the CSF avidity assay.
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