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Fish underwent a fasting period of between 24 and 36 hours prior to testing to control for any prior effects of satiation.
In Experiment I, salivary cortisol concentrations were additionally measured immediately before, during and after recognition testing to control for possible influences of circadian and awakening-related variations in glucocorticoid release known to influence memory function [34], [64].
Within the other two categories, all end points were secondary or exploratory at week 104, using a hierarchical approach to significance testing to control for multiplicity.
Subjects were satiated prior to testing to control for any differences in individual motivation driven by hunger, were never food-deprived in general and had constant access to water.
This requires researchers to adjust for multiple testing to control for type 1 error rate using various methods based on the family-wise error rate (e.g. Bonferroni corrections, Westfall and Young permutation), and the false-positive rate (e.g. Benjamin and Hochberg) that are under strict assumptions [ 70– 70].
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I used a partial mantel test to control for the association index matrix when comparing headbutting and mirroring to association at the group level.
Classifier accuracy and statistical significance were assessed using leave-one-out cross-validation and a random permutation test to control for over-fitting.
Gustatory response scores (GRS) were tested to control for effects of colony environments in the hive boxes bees were collected from.
Significance was set at p≤0.05 for the ANOVA, while a more conservative value of p≤0.01 was used for the follow-up tests to control for multiple pair-wise comparisons.
Parallel versions were used for memory tests to control for possible material-specific learning effects [ 16].
Nevertheless, we conducted additional tests to control for other less obvious potential effects of this bias.
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