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Cyclists have also become skilled at determining doses for other drugs that fall below testing thresholds but still improve their riding.
At second testing thresholds were enhanced in 14 of the treated patients.
Three genes, including SH2B1, MC4R and KCTD15, showed trends towards significance in the case/control association tests (P-value ranges from 0.015 to 0.065), but they did not pass multiple testing thresholds (based on number of genes tested).
This analysis is likely to uncover only very large effects with the present experiment given the smaller subclass sizes involved and the need for stricter testing thresholds to account for the increased number of multiple tests.
Threshold points for maximum change were noted after 4 h of exposure to 20,000 lux and 2 μM 9-cis retinal (Fig. 1), and confirmed widely reported testing thresholds of 2 h of exposure to 1mM H2O2 and 24 h of exposure to 100-400nM Staurosporine (Figs. 1, 2 and 3).
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Groups (6-OHDA and sham) were compared voxel-wise using a t test followed by a threshold-free cluster enhancement (TFCE) procedure with subsequent permutation testing, yielding a statistical map corrected for multiple testing, thresholded at p < 0.05 [16].
To evaluate the effectiveness/priority of this strategy for change detection, a set of testing threshold values from 1 to 20 at an interval of 1 were checked.
For future testing, threshold filters may provide a measure for artifact reduction strategies.
First, in this analysis, we primarily used alpha = 0.05 as a testing threshold.
The multiple testing threshold for the ratios using Bonferroni correction at nominal p-value of 0.05 is 1.18E-10.
This was done by defining the testing threshold as described by Pauker and Kassirer [see additional file 1] [ 12].
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