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Before testing the gene expression traits, we adjusted them for age, gender and PCs.
We further confirmed our model by testing the gene expression level of iNOS and p53 and p53 dependent target genes in VP16 or VP16-SXR transfected MCF-7 cells.
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We tested the gene expression of Nrf2 and its negative regulator, Keap1.
For most of the genes tested, the gene expression levels are significantly different between loj mutants and controls in both tissues (p < 0.05).
We first tested the gene expression of both the HGF ligand and c-Met receptor in PC-3 and DU145 cells.
To test the gene expression stability of the two markers, Q-PCR was performed using FRET hydrolysis probes in order to gain in specificity for subsequently analysis.
The geNorm applet for Microsoft Excel [ 72] was used to test the gene expression stability for the candidate genes (M = 0.595 for both 1f1α and bact), and their geometric mean was used to generate a normalization factor for each sample.
In addition to testing the two susceptible strains of M. domestica for differential gene expression, we further tested the gene expression values of the ALHF strain against an additional biologically-independent sample of the ALHF strain to ensure that the gene expression values were reproducible.
We further tested the gene expression in MCF-7 cells transfected with miR-24-2-specific antagomirs, and it was observed that inhibiting miR-24-2 expresultedresulted in significantly enhanced expression of BCL-2 and H2AFX compared to mock transfected control.
To test whether the gene expression signature shared by 5-NA and EerI was indeed a result of p97 inhibition, we knocked down p97 in 293T cells.
However, they used a different selection of chemicals (the semantic category "drugs" in the thesaurus) to test against the gene expression experiment.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com