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For testing the fraction of original reads mapping to the transcriptome assembly, Bowtie 2.1.0 [ 87] was used set to '--sensitive-local'. Identification of rRNA contigs to investigate contamination was as follows (although not poly-adenylated, rRNA is often sufficiently abundant in total RNA that it survives in RNA-Seq experiments prepared with poly-A purification).
Recent software provided both probabilities (normal and bootstrap) for three options related to the distance used for testing: the fraction of pairs, the standard deviations, and the fraction of range.
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The same analysis was used to test the fraction of aggression by the α-female.
We also tested the fraction of negative correlations dependent on the number of miRNA-binding sites in the genes.
We also tested the fraction of indirect TF-target pairs which could be connected by only using the relationships of the 25 TFs.
For all three conditions tested, the fraction of fluorescent cell pixels was about 50%188 h after seeding and increased as the culture progressed.
To test the fraction of diploid cells from a colony on selection medium, colonies were suspended in water, plated on rich medium, and 100 single colonies were transferred to selective media.
A Fisher-Exact test was used to test whether the fraction of genes in the Odom et al. data that are differentially expressed in response to HNF4α knock-down is significantly greater than the fraction of genes probed on our microarray that exhibit HNF4α-dependent expression, resulting in a highly significant p-value of p = 2.9 × 10-33.
For 11 of 179 (6.1%) tested samples, the fraction of mutant transcripts was estimated by two RT-PCR reactions because of assay failure in one of the three replicates.
Specifically, it tests whether the fraction of over- or under-expressed genes in each profiled sample includes a higher than randomly expected fraction of genes from one or more gene sets under analysis.
We built a 2×2 contingency table using the number of cancer-driver gene and non-cancer-driver genes, and tested whether the fraction of cancer-driver genes is significantly increased from SP to AP gene classes using Fisher's exact test.
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