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As described [ 13], we performed qPCR testing the expressions of these 10 genes in both cancer and matched normal tissues of the 10 patients in discovery screen.
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Under these conditions, we analysed cell responsiveness to N-activation by testing the expression of the fate determinant Tramtrack.
We first checked that the mouse model was working appropriately by testing the expression of the two Mecp2 isoforms described to date [7], [26].
We therefore analysed RPE differentiation by testing the expression of an RPE-specific marker, RPE65, five days after injection of LNT.shGFP, LNT.ZONAB or LNT.shZO-1 [18].
We verified the results by testing the expression of PSMB6 and EEF1A1 at five developmental stages in oocytes (GV, MI, MII) and embryos (D2, D3, D5).
We are currently testing the expression of predicted TR-proteins from N. vectensis.
Finally, when testing the expression of human xenografts we wanted to measure the effect of the host murine tissue.
The latter possibility was experimentally excluded by testing the expression of a plasmid-borne IND1 p.N271QfsX31 in the heterozygous strain ind1Δ /+.
The authors went on to confirm this conclusion by testing the expression of two reporter genes in Escherichia coli.
In this study, we tested the expressions of mitochondrial Bnip3 and cytoplasm Cyto C to explore the function of mitochondria.
We then tested the expressions of MTSS1 and miR-182 in paired normal liver and HCC tissues.
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