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Under these conditions, we analysed cell responsiveness to N-activation by testing the expression of the fate determinant Tramtrack.
We first checked that the mouse model was working appropriately by testing the expression of the two Mecp2 isoforms described to date [7], [26].
We are currently testing the expression of predicted TR-proteins from N. vectensis.
Finally, when testing the expression of human xenografts we wanted to measure the effect of the host murine tissue.
The authors went on to confirm this conclusion by testing the expression of two reporter genes in Escherichia coli.
The latter possibility was experimentally excluded by testing the expression of a plasmid-borne IND1 p.N271QfsX31 in the heterozygous strain ind1Δ /+.
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To examine whether higher glucose consumption resulted in even lower tendon-related gene expression, we tested the expression of three crucial transcription factors, including Scx, Mkx, and Egrl.
In addition, we tested the expression of the predicted target genes involved in MAPK and Wnt signalling pathways that were correlated in our discovery and replication cohorts.
To confirm the purity, we tested the expression of RPE markers and compared it to the expression of retinal cell type markers, including markers of photoreceptors (Crx, Nrl) that are in close contact with RPE.
We tested the expression of PD-L1 in tumor tissues of 62 patients by IHC staining, and found that smokers had higher membranous PD-L1 on tumor cells than nonsmokers (Fig. 4a).
Quantitative real-time PCR was performed to test the expression of HIF-1α and VEGF1 in HUVECs as well as IL-1β and TNF-α in RAW264.7 cell line.
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