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Auto-activators were identified by testing the activation of GAL1::HIS3 on minimal medium lacking leucine and histidine but containing 20 mM 3-amino-1,2,4-triazole (3-AT) in the absence of any AD-containing vector.
Simulated experiments are used to validate the convoy model, testing the activation of the different behaviors in the decision-making process.
In support of these data, RNA interference for Sonic Hedgehog (Shh), upstream activator of Smo, reduced BN stimulated growth, matrigel spreading and soft agar colony formation [ 7]. Surprisingly, when testing the activation of Gli transcription factor upon BN stimulation, we revealed the existence of a direct crosstalk between the two pathways.
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We tested the activation of these channels relative to depolarizing currents activated by elevated extracellular potassium as a calibration standard.
Therefore, in order to simplify our cotransfection assays, we decided to use protoplasts prepared from ttg1 mutant rosette leaves to test the activation of single-repeat R3 MYB genes by the GL3/EGL3-GL1 or GL3/EGL3-WER activator complex.
Our experiments that tested the activation of the human megalin promoter by luciferase reporter assay and the EMSAs, which used human megalin probes, were performed with mouse PPARα.
We tested the activation of PI 3-kinase in THP-1 cells stably transformed with a reporter construct specific for the kinase product phosphatidylinositol (3,4,5) triphosphate (PIP3) [53].
We tested the activation of p-CREB in the ACC of the pain memory model with IF and EMSA.
Specific antibodies were used to test the activation of the MAPK p38, NF- κB and I κB α.
To elucidate the molecular mechanisms of IL-1β-induced SYN expression, we first tested the activation of all three MAPK members, including Erk, JNK, and p38.
We further tested the activation of Akt and ERK1/2 in the presence of COD-B without or with cholesterol at short intervals.
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