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Between testing, samples were frozen at –80°C.
For serologic testing samples were frozen at minus 80°Celsius.
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The results showed a residual moisture content of 4.69±0.07% in all samples tested (all samples were frozen in IMT-2 solution).
All animals had undergone postmortem examination, and tissue samples were frozen until testing.
Samples were frozen in TEST buffer with 6% (w/v) glycerol and 10% (w/v) egg yolk as cryoprotectants, as previously described [54], and were kept in liquid nitrogen until further processing.
The samples were frozen at −70°C until testing was initiated.
The plasma samples were frozen at −20 °C until testing.
The serum samples were frozen at −80°C and tested later.
All samples were frozen at -20°C until tested.
All serum samples were frozen at -70°C until Widal testing was done in three batches.
All plasma and serum samples were frozen at −80°C until laboratory testing.
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