Sentence examples for testing promoter from inspiring English sources

Moreover, a testing promoter region is also characterized as previously explained in the section "Characterization of promoter regions".

This process created proxy datasets for testing promoter occupancy, allowing us to look for documented binding of the TFs in sites overlapping the 4,102 promoters, in the relevant cellular models.

By testing promoter activity of ten plant-specific promoters in five eubacterial species we could show that in 50%% of the tested combinations the promoter sequences could be used in the bacterial host [ 12, 13].

By testing promoter fragments corresponding to 0.5, 1.0, and 1.5 kb upstream of the tcu-1 and nit-6 ORFs, we determined that 0.5 and 1.0 kb fragments were insufficient to drive high levels of expression of GFP mRNA and protein.

Therefore, we split the testing promoters by expression level into five classes by RNA-Seq and predicted the S index for each subset by using the original model (Additional file 1, Figure S9).

For the self-activation test, promoter bait strains were grown on the SD/−Trp, -His (a synthetic Trp and His dropout medium) media in the presence of 0 mM, 10 mM, 30 mM and 50 mM 3-aminotriazole (3-AT).

As a test promoter we used the T. gondii ribosomal protein RPS13 promoter for which we provide experimental evidence of having a single major transcriptional start site, a condition favourable to the design of inducible expression systems.

To test promoter activity in rice, the OsNCED3 promoter, encompassing 2407 bp upstream of the ATG start codon of OsNCED3, and the OsNCED4 promoter, a total of 1939 bp upstream of the ATG start codon of OsNCED4, were amplified by polymerase chain reaction (PCR) and fused to a β-glucuronidase (GUS) coding region in the pCAMBIA1305.1 binary vector.

None of the tested promoter constructs showed an increased reporter activity due to c-MYC.

When compared to Nter-15Q, Nter-142Q led to an increase of luciferase activity for all tested promoter regions, with the exception of region A1.

Surprisingly, the expression of the c-MYC protein led to an inhibition of all tested promoter constructs independent of the selected serum concentrations.