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For testing on plants, cercosporin was extracted from PDA plates directly with water, which worked as well as extraction with KOH.
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The association of sanitizers with nitric oxide donors was tested on plant and human pathogens in order to measure to what extent synergistic effects occurred.
The mesh based methodology was tested on plant meshes reconstructed [ 23, 27] for a set of six plants studied at four time-points (i.e. 6×4 = 24 plant meshes).
We chose F. sambucinum strain T5 as a model for this study because it was the most aggressive strain when tested on potato plants, inducing a rapid wilting and yellowing that resulted in plant death (Figure S2).
Commercial formulations of A. nodosum have been tested on almond plants (Prunus dulcis [Mill.] D. A. Webb), which demonstrated increased growth and accumulation of K+.
The mutant strain was also tested on host plants of other rhizobial strains to check whether the mutation had an effect on host range.
Non-pathogenic Clavibacter strains from tomato seeds tested in the previous study [ 4] as well as other strains tested by Jacques and coworkers [ 3] did not introduce any disease symptoms when tested on tomato plants.
This approach effectively eliminated the problem of detecting "false positive" candidate markers (DNA bands appearing as candidate markers in the bulk, but proven as otherwise when tested on individual plants separately) [ 12, 13].
The model, written in the AMPL modeling language, has been tested on several plant test cases.
The derivatization procedure was then tested on four plant extracts: apple pomace, salvia sclarea (dried leaves and flowers), sea buckthorn (Hyppophae rhammnoides L). berries, and B. serrata resin.
The proposed methods are to test on the plant load shedding table from a paper pulp mill industry with 25 load points for practical applications.
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