Quality testing of the sequencing like depth of coverage (the a verage number of times a nucleotide is expected and observed to be sequenced) will not be explained here in details, but the importance of this step should be noted.
A four gamete test of the sequence variation around the centromere does not indicate recombination.
CR developed the sample barcode design, participated in the study design, suggested some tests of the sequencing method, annotated nuclear regions and helped to draft the manuscript.
In 18/34 cases where parental samples could be tested, all of the sequence changes identified had occurred de novo.
To eliminate any potential carry-over effects of previous experience of the tests, the sequence of testing in the VITI and LITI was counterbalanced across all experimental factors (Colony, Genotype and Time-of-Day).
CMV-PTTG-EGFP was then sequenced to test the authenticity of the sequence.
RFLP patterns with several enzymes guided the choice of those for sequencing and subsequently tested the accuracy of the sequence assemblies.
Range analysis of orthogonal test indicates the sequence of significance from high to low is as the second aging temperature, first aging temperature, second aging time and then first aging time.
In order to prevent the occurrence of systematic differences between the observers, due to repeated testing, the sequence of the observers was randomly allocated.
In all the test phases, the sequence of sections was the same (cervical, thoracic, lumbar).
