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In addition, a collection of 66 nontarget strains (66 species) were used for specificity testing of the array (additional file 1).
The GMAP presents an accessible solution because the obstacle of designing, constructing, and testing of the array as well as development of rigorous SOPs has already been overcome.
We used false discovery rate (FDR, Benjamini and Hochberg method) for multiple testing of the array data and genes having corrected p value less than 0.05 were selected for analysis.
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Most of these markers detected reliable polymorphisms across all accessions tested, while a subset of markers worked reliably only in a subset of diversity tested on the array.
Both types of hybridizations were used successfully in the testing of the arrays.
The crude amylase preparation obtained from test fungi under optimized conditions of SSF was further tested for the array of enzymes present in the crude enzyme preparation that were produced under the above-mentioned optimized conditions.
A total of 11,048 genes were called present out of 24,350 genes tested on the arrays and were used as the basis group for further analysis.
This manuscript describes the creation and testing of the SNP array and highlights its potential applications in Atlantic salmon genetics research.
For optimisation and sensitivity testing of the custom array, total RNA was isolated from ex vivo LPS-stimulated mouse peritoneal macrophages with the Aurum total RNA mini kit (BioRad), according to manufacturer's instructions, and RNA yield was measured using a NanoDrop spectrophotometer (Thermo Scientific, Waltham, MA, USA).
However the complicated design and intensive testing of the ZFN arrays might limit the scope of usage of this technology.
Tubular array structures are a very common component in industrial heat exchanging plant and the non-destructive testing of these arrays is essential.
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