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New advances in genomic technologies have led to parallel testing of multiple genes.
Because it allows testing of multiple genes at once, NGS greatly improves the variation detection rate.
Corrections were performed separately for each test to account for testing of multiple genes.
Corrections were performed separately for each test (e.g., GENECONV, NSS, etc.; TO, TLI/LM, etc). to account for testing of multiple genes (i.e., 2237 genes).
The application of massive parallel sequencing technologies for the testing of multiple genes simultaneously would be an efficient approach to molecular diagnosis in a subset of patients.
The massive amount of sequence data generated by a single NGS instrument can be harnessed to allow many permutations of concurrent testing of multiple genes in a number of patients.
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Delta CTs from qRT-PCR were compared using two sample t-tests and Holm's method [ 24] was used to control type-I error across tests of multiple genes.
These tests allow for simultaneous analysis of multiple genes (multiplex testing) or the entire genome at a cost that is often only slightly greater than a single gene test [ 20, 48].
Varying functional validation outcomes of multiple genes tested as miRNA targets (Figures 3B and 4) importantly demonstrate the complexity of target gene regulation by microRNAs and indicate the need for detailed validation in prediction- and inverse correlation-based studies.
To determine whether these vectors can be used to simultaneously inhibit the expression of multiple genes, we tested the ability of p3-siRNA to produce three different siRNA to target three different genes.
Next-generation sequencing enables the simultaneous analysis of multiple genes in a single test.
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