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First, the performance of SLAS largely depends on the linkage disequilibrium (LD) between testing loci and potential causal loci.
SLAS may have low power if the LD between testing loci and potential causal loci is weak.
However, in many other avian orders fewer microsatellite loci have been isolated and, therefore, the opportunity to develop microsatellite markers by testing loci from other species is limited.
To be conservative in selecting 25 candidates for testing, loci from the 56 contigs with putatively mitochondrial or chloroplast sequence were removed from the pool of candidates, leaving 209 primer sets for putatively nuclear microsatellite loci.
When, in some fields, the strategy of testing loci for association one-at-a-time led to disappointing results, the argument was frequently advanced that, since complex diseases clearly must involve interactions of causes in a mechanistic sense, association tests should allow for epistasis.
In conclusion, the strategy of testing loci identified in other autoimmune diseases, such as RA, for association with JIA is proving successful in the identification of novel JIA loci and is a complementary approach to the hypothesis-free methods of GWAS.
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The left panels of a and b show the primary structures of the TIGR/RGAP version 5 gene models for the two tested loci.
The five-color PowerPlex® ESX and ESI Systems allow co-amplification and detection of the current commonly tested loci, plus the five new recommended loci.
Interestingly, all genotyped individuals showed at least one triploid locus profile among the tested loci, which may be evidence of polyploidy associated to their life history, in particular to asexual reproduction by parthenogenesis.
Formation of H3K27me3 was not increased within these tested loci (Figure 3C).
Similarly, background DNA damage, revealed by the N+ immunoprecipitates remains virtually undetected for the three tested loci.
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