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The sequencing process was carried out according to the standard protocol of Illumina, including sample quality testing, library construction, library quality testing and library sequencing.
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The tested library also contained siRNA targeting EPHA2.
The isoindole polymerizes, resulting in colored derivatives and thus simplifying the identification of positive hits in the tested library.
The screening hit-rate was defined as the number of substrates divided by the size of the tested library.
Moreover, the largest contigs for each of the tested library types were considerably smaller than the largest contig generated from the unamplified control library.
When trying to predict the selectivity profiles of the tested library compounds, docking failed to give useful guidance (Supplementary Table S7).
(A ) Evaluation of the library insertion efficiency for four of the tested libraries shows up to 1,800 insertions per experiment.
Moreover, a range of open source projects such as Python [ 75], Perl [ 76], and Java [ 77] provide general purpose programming languages with rich and well tested libraries.
A specific bioluminiscence resonance energy transfer (BRET) assay (Xu, Piston and Johnson 1999) to test libraries of peptides that could disrupt the T A interfaces was developed (Nieto et al., 2006).
The same formulas were applied to the 24-nt PHAS windows in all tested libraries, with a change of the phase register length from 21 nt to 24 nt.
To facilitate routine conduct of such testing, libraries of cell lines from many different subgroups (infants, the elderly, asthmatics, diabetics, etc).
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