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Rare-Variant association testing for sequencing data was carried out to detect rare mtDNA variants involved in human longevity.
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Phylogenetic analyses were used to confirm the morphological identification of all strains and to test for sequence divergence.
MITE sequences that are either less than 50% or greater than 75% similar to the corresponding O. sativa MITEs were identified to test for sequence divergence of MITEs within Oryza.
In the case of maritime pine, no sequence database was available to test for sequence redundancy.
We then tested for sequence saturation using both the Steel (for amino acids; [21]) and the Xia (for nucleotides [22]) methods [55] using DAMBE [56].
The same 33 clones showing exon skipping enhancement on the hE72-Luc construct were tested for sequence specificity against the hE40-Luc construct.
To select the amiR-53R that has the best inhibition efficiency, the amiR-53Rs were initially tested for sequence-specific silencing on the target 53R gene by employing transient transfection of a plasmid expressing 53R (pEGFP-N3-53R).
DNA samples positive for C. burnetii IS1111 were tested for sequence type (ST).
Clones were tested for sequence representation from different ends of the reported 10 kb DRIM mRNA (RefSeq: NM_014503).
The RNA oligonucleotides were designed to test for sequence-specific binding and to avoid the possibility of base pairing.
Metastats was used to test for sequences that were differentially abundant in KD cases and controls [ 12].
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