After in vitro expression, mutants were tested for functionality.
Software systems are often engineered and tested for functionality under normal rather than worst-case conditions.
Some test scenarios, e. g., 1 or 7, are benign scenarios to test for functionality and connection.
After encapsulation, the islets were tested for functionality using the dynamic perifusion procedure with low- and high-glucose concentrations.
The application was tested for functionality in the field and for its ability to match Munsell notation values determined using a Munsell Soil Color Chart (MSCC).
When tested for functionality on several varieties of common wheat (Triticum aestivum) including Chinese Spring, 64 of these 78 primer pairs were found to be functional.
To test for functionality and a potential contribution of an IP3-releasable Ca2+ pool to the modulation of Ca2+-handling in hiPSC-CMs we first examined the expression and localization of the IP3R at the protein level.
The insertion was confirmed by sequencing and the VavlacOVenus (VLV) construct was tested for functionality by transient transfection into 293T cells, either alone or together with a plasmid encoding the codon optimized mammalian version of the lacI [10].
Full-size antibodies extracted and purified from plant tissues were tested for functionality and specificity by (i) binding to HER2/neu on the surface of a human mammary gland adenocarcinoma cell line, SK-BR-3, in fluorescence-activated cell sorting assay and (ii) testing the in vitro and in vivo inhibition of HER-2-expressing cancer cell proliferation.
Each vector described in Figure 4 and Table 1 was also tested for functionality of the fusion protein (V5, FLAG, GFP, NTAP, FKBP12DD, V5-Ubc9, GST, myc and nuclear targeting), confirming epitope detection by immunoblotting or immunofluorescence (Figures 6 8 and Supplemental Figures S1 and S2).
