The GM maize grown with Roundup was as in usual agricultural practice, and maize was tested for contaminating pesticides.
Prior to synthesis of Q-PCR template cDNA, RNAs were tested for contaminating genomic DNA.
Conformations used in all assays were tested for contaminating LPS by QCL-1000 Chromogenic LAL Endpoint Assay (Cambrex).
Negative controls included using each set of primers on the RNA to test for contaminating DNA and on a no target reaction mix.
Two of the three biological replicates used for the microarrays were used in the reverse transcription and quantitative PCR. 1 μg of each RNA was used in reverse transcription reactions with either 3.75 units of Thermoscript (Invitrogen, Carlsbad, CA) or no reverse transcriptase as a -RT control, which was tested for contaminating genomic DNA in PCR reactions.
Indeed, it is not possible to identify precisely individuals who were exposed to SV40, as few vaccine batches were tested for contamination, not all batches were contaminated and distribution of contaminated batches from one country to another was not controlled (Gazdar et al, 2002).
Cell lines from these sources are tested for identity and contaminating microorganisms that commonly occur in culture, so they are unlikely to be contaminated or misidentified, unless so stated in the accompanying literature.
Specific primers (Sigma) for amplification of hemoxygenase-1, hypoxia inducible factor 1-α (HIF-1α), interleukin-6 and -8, TNFα and complement component C3 specific cDNA were designed (Table 1) using the Primer Express software package (Applied Biosystems, Foster City, CA) and tested for amplification of contaminating genomic DNA.
Fourth, the general method for testing a live viral vaccine strain for contaminating viruses is to neutralize the vaccine virus and test for adventitious viruses both in vitro and in vivo.
Assessment for contamination through learning effects was undertaken by testing for a difference in the intervention effect for the primary outcome across naive and potentially contaminated periods.
