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Primers used for testing expression of the U2, U4 and U6 snRNA candidates are listed below: Thanks to Errol Kwan from MicroAquatech for supplying Giardia cell culture.
However, when testing expression levels from early stages versus late stages, these were found to be significantly different in all cases (Supplementary Table S5).
Have the authors considered using a longer 'waiting' period between development and testing expression?
Also, we did not analyze other immunohistochemical subtypes by testing expression of MUC1, MUC2, etc.
As proof of principle we demonstrate the functionality and efficiency of this system by testing expression of several cDNA and shRNA sequences in a number of cell lines.
Finally, to assess the quality of the whole process, each population isolated must be characterised by testing expression of cell-type specific markers and in vitro and in vivo functional assays [ 10, 11].
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We tested expression of Turbo, tdTomato, mCherry, mKat and mPlum expressed from Phsp60 or Psmyc.
We further tested expression of GH receptor in protein lysates of testes (Figure 8B), ovaries, thymus, and adrenal glands (data not shown).
One ml of culture was removed every 24 h to test expression levels using the synthetic 4-nitrophenyl β-xylopyranoside (PNPX) as described in section 2.4.
RNA was isolated from brain, liver and skeletal muscle with Trizol reagent (Invitrogen) to test expression.
In addition to PGCs, we tested expression of retrotransposons in 3 days post partum (dpp) spermatogonia.
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