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In this article we introduce an in vitro model for hepato-mediated toxicity testing consisting of a Hepatocyte-Bioreactor connected to a microphysiometer system for monitoring of the extracellular acidification rate (EAR) of cells.
A touchdown thermal profile was used for initial testing consisting of a 3-min denaturation at 94°C; followed by 12 touchdown cycles at 94°C (30 s), 66°C (40 s) (−3°C/second cycle), 72°C (1 min); 30 cycles at 94°C (30 s), 48°C (40 s), 72°C (1 min); and final extension at 72°C for 20 min. One locus performed better at a fixed annealing temperature of 55°C (Table 1).
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Testing consists of a series of numerical experiments combined with visual assessments on a set of 400 3 km-scale landscapes.
GMMs with diagonal covariance matrix were built for every category while testing consisted of a simple comparison of log-likelihoods.
Testing consisted of a set of six emergency medical scenarios for each participant, each with pathology that was potentially detectable by PoCUS.
Testing consisted of a 10 s accommodation period followed by a 2 s stimulation of the antenna similar to that of training.
Testing consisted of a 30 min exploration period.
Testing consisted of a questionnaire, blood pressure measurement, and drawing of a blood sample to identify any confounding factors.
In ∼40 different datasets we tested, consisting of a variety of ChIP-Seq, methylation and control/input DNA, the phantom peak occurred with varying strengths.
The US system we tested consists of a 5-13 MHz linear probe connected by wireless technology (8 GHz ultra wide band) to a compact, keyboardless device.
The vehicle tests consisted of subjecting a sport utility vehicle to three different ramp slopes.
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