To test whether the loss of checkpoint activity in the Mad1-RLK/AAA or Bub1-cm1 mutant (Fig 1F and L) is at all related to the loss of Mad1 from kinetochores, we tested checkpoint activity after artificially recruiting Mad1 to kinetochores through fusion to the kinetochore protein Mis12 (Fig 2).
Johnson said there would be more random covert testing at checkpoints.
More than a half-dozen luggage manufacturers, among about 60 that initially responded to a T.S.A. request for proposals about three months ago, have submitted prototypes for testing at checkpoints at three airports: Dulles, outside Washington; Austin-Bergstrom in Texas and Ontarioo, near Los Angeles.
After the report, Homeland Security Secretary Jeh Johnson reassigned acting TSA administrator Melvin Carraway and said there would be more random covert testing at checkpoints.
Johnson also ordered TSA to take measures to reform security by revising protocol, retraining staff, retesting airport screening equipment and conducting more random testing at checkpoints.
Consequently, a chk1 deletion strain renders the cdc17-K42 strainviableble at 32°C and can be exploited to test the checkpoint function of chk1 alleles [26].
Thus, we tested ATR/Chk1 checkpoint activation by using a phosphospecific antibody that recognizes the ATR-dependent phosphorylation of S345 in human Chk1 in response to replicative stress, for example, UV irradiation or HU treatment (Zhao and Piwnica-Worms, 2001).
As overexpression of Cdt1 has been shown to induce DNA re-replication followed by head-to-tail collision of replication forks, we tested whether checkpoint activation occurred in CLL cells under these conditions.
As Dot1, the H3-K79 methylase, is important for activating Rad9 in response to DSBs (Giannattasio et al, 2005; Wysocki et al, 2005; Toh et al, 2006), we tested the checkpoint role of Dot1 after telomere uncapping.
He said both had been tested at checkpoints to ensure that they met government specifications.
Vehicle seizures in the Bay Area have been more common than sobriety tests at checkpoints during the past decade.
