We have not tested body weight and glucose homeostasis in cohorts of Ins1CreERT2 mice.
As was done previously [ 10] for the three physical activity traits, we first tested body weight (WT) for potential effects due to sex, litter size, and rearing block.
Prior to the test, body weight (BW) measurements were taken with an electronic scale.
For Rane's test, body weight and temperature were measured before infection and from day 3 7 after infection.
> -wrap-foot> aSamples collected before the age of 7 days bSamples collected at the age of 7 days or after c1st test: Body weight = Birth weight.
Vital signs, adverse events, laboratory tests, body weight, 12-lead electrocardiogram (ECG) and pregnancy test will be performed for the purpose of safety evaluation.
Pleiotropic or closely linked QTL associated with average daily gain and mid-test body weight were detected on BTA 6 at 38 Mb and BTA 7 at 93 Mb confirming previous reports.
The safety measures were adverse events, drug-related adverse events, general laboratory tests, body weight, vital signs, 12-lead electrocardiogram, and injection-site reaction, and were assessed until day 15.
Animals were observed for up to 14 days for the acute toxicity test and 28 days for the subacute toxicity test for any sign of toxicity (behavioral change related to central nervous, cardiovascular and gastrointestinal systems as well as complete blood count, liver and kidney function tests), body weight change and water and food consumption.
The pleiotropic or closely linked QTL on BTA 7 at 23 Mb identified in the Angus population harbours a promising candidate gene ACSL6 (acyl-CoA synthetase long-chain family member 6), and was the largest effect QTL associated with dry matter intake and mid-test body weight explaining 10.39% and 14.25% of the additive genetic variance, respectively.
