Immediate, blinded, and systematic color Doppler examination of each pair of testes was recorded before and after IV administration of 1 ml (300 mg) SHU 508, a gas-containing microparticle.
Fork length, wet body mass, condition factor (fork length/body mass), and gonadosomatic index (GSI; testes mass/body mass) were recorded.
At sacrifice, gross observations of the organs were recorded, and the testes, kidneys, spleen, liver, lungs, and brain were all carefully removed and weighed.
The NB and IC defects as well as the total numbers were recorded from 10 lat B treated testes.
The laser Doppler flow signals were recorded for at least 5 min on the surface of both testes and average blood flow and vasomotion frequency were later analysed using the software Perisoft for Windows 1.01 (Perimed AB).
The both testicular weights were recorded and the gonado-somatic index was calculated with the ratio of testes weight and body weight.
Using a dissecting microscope (SZX12, Olympus Corp., Tokyo, Japan) and dissecting tools, testes, seminal vesicles (SV), and ventral (VP), dorsal (DP), lateral (LP), and anterior (AP) prostate lobes were dissected from PND28 and PND56 animals and wet weights were recorded.
The proportion of individuals with well-developed gonads (ovaries and/or testes – B. improvisus is an hermaphrodite) or fertilized eggs was recorded at the end of the experiment.
Moreover, the wet weight of the kidneys, spleen, liver, adrenal glands, pancreas, lung, heart, thymus, testes, epididymides, uterus, ovaries and brain of all animals was recorded.
Accessory glands and the uncoiled testes were dissected out in phosphate buffered saline on a glass slide and the length of the line that bisected the middle of each organ was recorded [ 13].
