Sentence examples for testes using a from inspiring English sources

Twenty microliters of a mouse or human GF-derived cell suspension (0.2 0.5×106 cells/testis) in cold Hank's balanced salt solution or DMEM/F12 (Invitrogen) were injected into the medulla of mouse testes using a Hamilton syringe.

RNA was extracted from 20 pairs of dissected testes using a guanidine hydrochloride, phenol/chloroform method [ 34], and DNAase treated to remove DNA contaminants (New England Biolabs).

Round spermatids were purified from WT and miR-dKO adult testes using a mini-STA-PUT method (Ro et al., 2007; Song et al., 2009; Wu et al., 2012).

Individual seminiferous tubules were isolated from testes using a collagenase and hyaluronidase method, and Trypan blue positive tubules were transferred into a drop of PBS + with 0.2% BSA in a live-cell chamber overlaid with PBS-saturated mineral oil.

Male germ cells were isolated from testes using an adapted version of the STAPUT method [22].

After performing western blot analysis of total testis lysates, RBM44 protein was shown to be decreased in Rbm44 heterozygous testes and absent in the Rbm44 homozygous testes using an antibody generated against RBM44 amino acids 471 609 (i.e., N-terminal to the deletion)(Figure 8G).

We found no evidence of consistent up- or down-regulation in liver, kidney, or testes using any gene set combination reported to date, including the set of bound genes by E2f4 in mouse.

Western blot analyses showed significant differences in the expression of MC3R and MC4R in the lizards' brain and testes, using actin as an internal standard.

Since predictor variables were related to each other (thus non orthogonal), they were added to the multiple regression analysis in the following order: body mass, testes mass, using a sequential (Type I) sum of squares.

In the current study, we identified the putative SSCs and their niche by identifying the LRCs in zebrafish testes and using a transgenic zebrafish expressing enhanced green fluorescent protein under the control of the germ cell specific vasa promoter (vasa::egfp) [25].

In this case, since predictor variables are related to each other, they were added to the multiple regression analysis in order, first body mass, and then testes mass, using a sequential (Type I) sum of squares.