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Now a skilled urologist can retrieve sperm either by siphoning the epididymis, the coil that links each testis to the vas deferens, or by mining the testes using hollow needles or open surgery.
RNA was extracted from 4 week-old testes using the RNeasy Mini Kit (Qiagen, Valencia, CA).
Male germ cells were isolated from testes using an adapted version of the STAPUT method [22].
Next we decided to investigate AFP for expression in the testicular cells of EC 14 Ds testes using immunohistochemical techniques.
Total RNA was extracted from testes using the RNAqueous®-Micro Kit (Ambion, Austin, TX, USA, http://www.ambion.com).
To assess whether rSey2/+ rats display autistic phenotypes, we executed several behavioral testes using only male rats to avoid potential influence of menstrual cycles except for USV test.
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The 55 μl of sperm suspension of the left testes used to fertilize the eggs contained thus approximately 8.3 × 106 sperm (median; range 1.0 × 106 – 64.4 × 10).
Testes used in this study were not obtained in the context of animal experiments, but collected as remnant material from specialized veterinary clinics immediately after dog castration surgery was performed using the standard procedure (adhering to best practice of veterinary care) of that clinic, at the owners' request, and not for the purpose of the experiment.
Treatment for most orchitis is administration of antibiotics, bed rest, support of the testes, use of compresses, and surgical relief or drainage when required.
Meticulous comparison of the two testes by using transverse views is mandatory in these cases.
Since predictor variables were related to each other (thus non orthogonal), they were added to the multiple regression analysis in the following order: body mass, testes mass, using a sequential (Type I) sum of squares.
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