The paired testes consist of a series of seminal tubules that form primary spermatogonia (immature spermatozoa) at their upper ends.
Testes consist of somatic and pre-meiotic germ cells, meiotic spermatocytes and post-meiotic spermatids and each of these cell types contains numerous subtypes that have their own characteristic gene expression profiles [ 1, 25].
Our replicate transplantations consisted of OSKMV cell injections into the seminiferous tubules of an additional six testes with subsequent IHC analysis of serial cross sections regardless of tissue structure.
The DCE-MRI scan consisted of a 2D T1-weighted multi-slice multi-echo scan to acquire contiguous axial images across the entire testes.
Hybridisation buffer consisted of 5× SET (0.75 M NaCl, 5 mM EDTA, 0.1 M Tris [pH 7.8]), 10% dextran, 0.2% BSA, 0.1 mg/ml polyadenosine, 20 μg/ml salmon testes DNA and 0.02% SDS.
The mature testes of medaka consist of many cysts containing differentiating spermatogenic cells (spermatogonia, spermatocytes, spermatids and spermatozoa), and spermatogonial stem cells (SSC), each wrapped by one Sertoli cell, forming a niche.
The male organs consist of testes, from which extend numerous tubules (vasa efferentia) that unite to form a sperm duct (vas deferens); the latter becomes an ejaculatory duct through which sperm are released to the outside.
The initial part of the reproductive tract of S. littoralis consists of testes that are separated from paired UVD by the epithelial barrier.
SAGE libraries were constructed from the total RNA isolated from both testes of a single male (TT 1) and from the pool consisting of equal weight amounts of total RNA isolated from both testes of three male littermates (TT 2).
Since we implemented these experiments on testes harvested from the mid-pupal stage, they consist of spermatocytes at various stages of ciliogenesis.
