Meticulous comparison of the two testes by using transverse views is mandatory in these cases.
Another four miRNAs (mmu-miR-34b, mmu-miR-122a, mmu-miR-16 and mmu-miR-101) were confirmed to be differentially expressed in mouse testes by using conventional Northern blot analysis [2].
Total RNA was isolated from testes by using the automatic DNA/RNA extractor (BioRobot M48 workstation; Qiagen; CA, USA) and first strand cDNA synthesis was conducted on 0.5 μg total RNA using SuperScript™-II Rnase H- Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA).
Spermatocytes and round spermatids were purified on the day of testes collection by using collagenase and trypsin treatment, followed by sedimentation at unit gravity (StaPut procedure) [ 87].
In this work, we demonstrated, by using salmon testes DNA (2000 bp), poly- L-lysine) (poly- L-lysinely(ethylene oxide)-b-poly- L-lysine) (PLL35-b-PLL35), thandthe DNA compoly ethylene in dimethylfoxide -b-Poly- L-lysineer than thoxide -b-Poly- L-lysineoxide -b-Poly- L-lysine
As a last metric, we obtained all sex tissue-specific genes (those primarily expressed in testes, accessory glands, or ovaries) by using the following formula to calculate the tissue specificity index, τ, for each gene: where N is the number of tissues, E_i is the expression in tissue i, and is the maximum expression of the gene in all tissues (Yanai et al. 2005; Larracuente et al. 2008).
The relative expression of each gene comparing Wolbachia-infected testes to uninfected testes was normalized against the reference gene (rp49) by using the 2-ΔΔCT calculation method: ΔΔCT = (CT, Target-CT, rp49) w Mel- CT, Target-CT, rp49) w Mel- CT 46].
Eggs were manually stripped from the females and fertilized in vitro in 0.3× modified mammalian Ringer's solution by using sperm obtained from the dissected testes of the three males.
To test this hypothesis, we used the testes culture system developed in our laboratory to inhibit histone acetylation or promote premature histone acetylation by using specific inhibitors (Awe and Renkawitz-Pohl, 2010) and analyzed the methylation status of H3K79me3 in treated testes.
We demonstrate such a developmentally regulated process by using carefully staged cells (SC, RS, ES, and Ed) from mouse testes.
Line sitters, copy sellers, testes: All of this material suggested ways the elusive quality of truth can be pursued (or claimed) by using the human body.
