Equivalent amounts (1 to 2 μg) of labelled tester and control samples with similar dye incorporation efficiencies were pooled, lyophilized, and hybridized to microarrays as previously described [ 35].
For example, for a gene up-regulated 2-fold in tester over control, the formula is (a + 2b)(a' + 2b') = aa' + 4bb' + 2a'b + 2ab'.
cDNA fragment pools resulting from subtractive hybridization representing toxin-induced mRNAs ("exposed" pool as tester and "control" cDNA as driver) or for toxin-repressed mRNAs (reciprocal experiment) were further enriched with a "suppression PCR" step.
Equivalent amounts (1 to 2 μg) of labelled tester and control samples were pooled, lyophilized, and then re-suspended in 35 μl of hybridization buffer [1 × DIGEasy hybridization solution (Roche, Laval, QC); 0.5 μg/μl of torulla yeast tRNA].
Equivalent amounts (2 μg) of labeled tester and control samples were pooled, lyophilized, and then re-suspended in 42 μl of hybridization buffer [1 × DIGEasy hybridization solution (Roche Applied Science); 0.5 μg/μl of Torulla yeast tRNA (Invitrogen); 0.5 μg/μl of salmon sperm genomic DNA (Invitrogen)].
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Coevolved females appeared to be less attractive to our tester males than control females, as coevolved females were mounted less often than their control counterparts.
After 1567 he became control tester of coins at Kutná Hora, near Prague.
The ratio of tester signal to control signal for each gene was transformed to its base 2 logarithm [ 42], log2 [Tester Signal/Control Signal], referred to as "Log Ratio".
The ratio of tester signal to control signal for each gene was transformed to its base 2 logarithm [ 89], log2 [Tester Signal/Control Signal], and is referred to as "Log2Ratio".
Two rounds of hybridization were performed using 48A9 cell cDNA as the tester and ground control cell cDNA as the driver.
