Sentence examples for tester cell from inspiring English sources
This screen identified 68 compounds, which were further characterized for their effect on ERK inhibition using a tester cell line in which luciferase expression was under the control of activated ERK.
These same cell types formed a colony within 2 days in the absence of the tester cell lawn.
Utilizing two tester cell lines as relevant models for the envisioned ectopic or oral treatment and applying several different cell biological assays, proved to be a suitable combination of screening tools.
A reconstruction was done in which 100 cells of a doubly mutant revertant (either mDEL1, purR or mDEL1, purO) were plated on selective medium either alone or together with 10 tester cell.
In Figure 6D, the colonies derived from added doubly mutant cells all appeared on day 2, while the revertant colonies derived from the tester cell population appeared later and presumably arose from pre-existing singly mutant cells in the tester population that required time and a second mutation before they could make a visible colony.
Prior to the panning process, we constructed the BCG-specific γδ TCR-transfected cells as tester cells and non-specific γδ TCR-transfected cells as driver cells.
Therefore, during the screening procedure, we did not guarantee that all peptides that partially bind driver cells and tester cells could be excluded.
The tester cells and bound phages were both incubated with E. coli host strain ER2738 and the phages were rescued by infection with bacteria when the cells died.
To determine the input ration of wild type : tester cells, the mixes were titered on both TRP (selective for wild type) and ADE (selective for tester strain) dropout media.
The cells were then pelleted by centrifugation at 800 rpm for 5 min. The supernatants containing the phages were incubated with the blocked tester cells for 1 h at 37°C before the cells were pelleted again.
The tester cells and driver cells were seeded into 96-well plates (1×105 cells/well) one day before use and then fixed in 96-well plates, with 4% formaldehyde for 15 min at room temperature.
