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Each sample was hybridized and tested as duplicate samples.
Experiments were repeated three times independently with duplicate samples.
Performance of this assay at Quest Laboratory has been extensively tested in prior NHS study samples with hormone stability studies, test retest studies, testing duplicate samples, and embedding samples with known values within studies.
Genotyping quality control was tested using duplicate DNA samples within studies and SNP assays, together with direct sequencing of subsets of samples to confirm genotyping accuracy.
We averaged the FPKM from duplicate samples.
We included four duplicate samples to test the reproducibility of the whole approach.
Individual DNA samples were tested, in duplicate, with a previously described TaqMan assay with a lower limit of detection of 1 C. burnetii organism (8 ).
Briefly, we used duplicate samples with positive and negative controls.
All samples were tested in duplicate with the means of the total copy and mutation-specific CTs used for the determination of the ΔCT.
All serum samples were tested in duplicate with a concurrent positive control and a minimum of four negative (Dutch blood bank donor) controls per plate.
All serum samples were tested in duplicate with viral and negative control antigens [ 21, 22].
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