Each variant was assessed in skeletal muscle from the patient, whereas blood, urine, fibroblasts, and buccal mucosa were also tested where possible (Table 1).
Parents of patients with a mutation were tested where possible with direct sequencing of the mutated SCN1A region or multiplex ligation-dependent probe amplification.
Samples without β-globin were re-tested where possible a second time and rendered invalid if β-globin DNA was not able to be amplified.
This study was not designed to test the RCP-3Qs but to look at how poor control was being defined and operationalised in primary care and to test, where possible, the strength of each assessment model for predicting poor control of asthma.
Our detector from [ 12] also improves the method in another way, by using a quadruplet test where possible.
The results highlight the influence of the assembly and annotation pipeline on comparative content and function analysis, and emphasize the need for contributors of genomic data to provide complete information about the analytic provenance of their assembled and annotated genomes, and for consistent workflows, justified by benchmark testing where possible, to be used throughout a project.
Normality tests were performed; if the test failed, where possible standard transformations such as square root, reciprocal or logarithmic transforms were used to generate a Gaussian distribution.
To reduce the number of test animals, therefore, programs such as the EDSP have made increasingly attempts to include in vitro methodologies that are based on stable cells lines or sub-cellular compartments and modeling approaches (e.g. QSAR) in their testing scheme where possible.
Samples tested as positive by the commercial NAATs test were genotyped, where possible, by a MLVA-ompA sequencing technique.
We tested for recent population constrictions using Bottleneck 2.2 [ 33]. Isolation by distance tests were performed where possible using IBD v 1.52 [ 34].
Other less common causes of pneumonia (ie, Chlamydia psittaci, Legionella, and Staphlycoccus aureus) should also be considered and tested for with serological tests, urinary antigen testing, and microbiological culture where possible.
