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Concurrently to characterization analyses, the kinetic parameters of the Mars and Van Krevelen (MVK) model, representing well the rate of consumption of isobutane over the solids tested were determined by varying partial pressures of isobutane and oxygen.
Fold increases or decreases in each miRNA level of cell lines tested were determined by reference to the level of AZ-P7a cell line.
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The route tested was determined by readily available online tools, such as Google Maps, and evaluated based on judgment as to the route that was most likely to be selected by knowledgeable drivers.
In the absence of published resistance breakpoints for Aeromonads, tolerance to the antimicrobials tested was determined by examination of the frequency distribution of minimum inhibitory concentration (MIC) and disc diffusion diameter values for all the 127 isolates examined.
The stability of the mutagenic activity of the samples tested was determined by repeated analysis using the Ames Salmonella assay.
The TTO activity against the C. albicans strains tested was determined by broth macrodilution using the general dilution standards as described by PN-EN ISO 20776-1 2007 [ 24].
The fluconazole activity against the C. albicans strains tested was determined by broth macrodilution using the general dilution standards as described by PN-EN ISO 20776-1 2007 [ 24].
Cell viability under the various conditions tested was determined by the addition of resazurin to a final concentration of 50 μM and incubation for 5 h.
Comparative sensitivities of PCR tests were determined by testing log10 dilutions of plasmids with inserts containing corresponding provirus sequences.
The results were tested by ANOVA and optimum conditions for a verification test were determined by statistical calculations.
Correlations between UCS and results of each above-mentioned tests were determined by a univariable regression analysis.
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