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The investigation reveals that the best tested variant of MDE outperforms the original MDE itself, but also that the best variant depends on some properties of the function to be optimized.
Inhibition of MEK with PD98059 did not affect tumor growth in any tested variant.
It is also interesting to note that the large stability difference between F3-GFP and F2-GFP (Fig. S6) is caused by a single-substitution (F130V in the tested variant, F130L and F130I in the other two selected variants).
The tested variant in CYP2E1 is in the regulatory region and is associated with an increased transcriptional activity (Hayashi et al. 1991).
We tested variant proteins for loss of function by enzymatic assay and mice lacking GCS function were generated, to test the effect on embryonic development.
The induced cell death displayed signs typical for apoptosis (Supplementary Figures S2A and B), and was significantly reduced in all tested variant cell lines compared with WT cells.
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Comparison of the energy effectiveness of gas suction indicated superiority of the single-orifice nozzles with swirl inserts over the other tested variants.
In silico PT has several advantages over the use of physical samples, including greater flexibility in tested variants, the ability to design laboratory-specific challenges, and lower costs.
The tested variants showed high total turnover numbers in the order of 4000 6000 and can be used as biocatalysts for preparative scale synthesis.
During this benchmark it became apparent that the virtual screening performance of all the tested variants of USR is highly dependent on the query molecule and the compound library that is screened.
Transmission disequilibrium test (TDT) analysis did not find evidence for unequal allelic transmission ratios at any of the tested variants in the combined sample (data not shown).
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