The Infectious Diseases Unit of the Federal University of Espírito Santo performed both tests using kits supplied by the Brazilian Ministry of Health.
Data were collected during two cross sectional studies, where cats were tested using IDEXX FIV/FeLV ELISA kits.
All samples positive for the first ELISA test were tested using Genedia kits (Greencross Lifescience Corp ,Kyunggi-do, South Korea).
Albumin level was tested using ELISA kits (Rat Albumin ELISA Quantitation Set, E110-125, Bethyl) according to the operation manual.
Serum samples were tested using ELISA kits for interleukin-1β (IL-1β) (R&D Systems GmbH, Wiesbaden, Germany) according to the manufacturers' instructions.
Both cameras were tested using their kit lenses and processed in Adobe Lightroom.
The activity of amylase, trypsin and lipase were tested using commercially available kits (Nanjing Jiancheng Bioengineering Institute) in accordance with the instructions as indices for intestinal digestion capacities.
Using a candidate gene approach, ITPA variants rs1127354 and rs7270101 were tested using the ABI TaqMan kit.
The porcine sera were tested using the HEV Ab-ELISA kit (Axiom).
Serum insulin was tested using radioimmunology assay kit (North Institute of Biological Technology; Beijing, China) using a XH-6020 gamma counter (North Institute of Biological Technology).
The membranes were tested using western blot kit (Invitrogen, USA) according to the manufacturer's instructions.
