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We selected a number of genes that had roles in transcriptional regulation, development and signal transduction and tested the expression level of these genes using quantitative RT-PCR.
Therefore, we tested the expression level of miR-29a using the hepatoma cell lines which were reported with different metastasis potential as a model, such as MHCC-97H and MHCC-97L [24].
To further confirm that hsf1-R206S, F256S cells had enhanced basal activation of Hsf1 at 25°C, we tested the expression level of numerous known transcriptional targets of Hsf1 in these cells.
Since previous yeast two hybrid results indicated possible interaction of SNAP29 with other members of the EHD family [12], we also tested the expression level and intracellular localization of other EHDs in the absence of SNAP29.
We also tested the expression level of NRGN in these samples by performing qRT PCR.
Since cross-talk between EGFR and IGF-1R exists we tested the expression level of IGF-1R in these lines.
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Therefore we independently tested the expression levels of a panel of mitotic regulators that were identified as differentially expressed in the H' vs H analysis.
Therefore, we tested the expression levels of these two genes in their most functionally relevant tissues: SALL1 in kidneys and PTEN in the bone marrow.
We tested the expression levels of 12 randomly selected genes by qRT-PCR analysis (six specifically deregulated genes were chosen on each microarray list).
We tested the expression levels of probes that overlapped with endo-siRNA reads.
We first tested the expression levels of canine BRCA2 in mammary gland and mammary tumor samples by qRT-PCR.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com