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Enzyme reuse was tested performing three different treatments before each batch: washing the enzyme system with either n-hexane or water, or suspending the immobilized enzyme in water for 24 h.
In order to establish the amplification conditions, different concentrations of primers and probe were tested, performing the reactions in a 20-μl volume, containing LightCycler® 480 II Probes Master (Roche Diagnostics), 0.8 μM of each primer, 0.2 μM of Taqman probe, 5 μl of isolated genomic DNA and PCR grade sterile water.
All other variables were tested performing an analysis of variance (ANOVA) with replicate and ploidy as fixed effects.
However, the predictive value of this indicator was not tested performing a volume challenge and checking the effects on cardiac output (CO) or stroke volume.
Interaction between QTL and environmental conditions was tested performing the QTL analyses using three different time-points of measurements corresponding to different water irrigation regimes.
The significance of assumption was tested performing 1000 class permutations and observing the diagram distribution of the red classes (see Additional file 1).
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Hundreds of actresses between 15 and 28 auditioned, and those with the right presence were screen-tested, performing several prepared scenes in period costumes and makeup in a television studio.
This balance is for tests performed.
But DNA tests performed for the prosecution are seldom reanalyzed.
The I.O.C. conducted 4,770 doping tests in Beijing, the most drug tests performed at an Olympics.
Tests performed as part of Dr. Berrill's assessment measured his I.Q. at 76.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com