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We tested one plate containing 384 compounds per day using SPR.
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Further, the sample cDNA and the corresponding reference cDNA were tested on one plate, so that the data can be compared directly.
Each gene was tested with both cDNAs and the cDNAs were also tested in duplicate on one plate.
One hundred such colonies were randomly chosen and were tested on LB plates containing 100 μg ml-1 ampicillin.
For intra-assay CV, the samples were tested 20 times on one plate.
Control serum was tested twenty times on one plate to assess within-assay CV, which was found to be 6.2%.
R&D Systems reports an intra-assay coefficient of variation (CV) of between 2.9 and 3.9%, as determined by three samples of known concentration tested 20 times on one plate.
Using a 10-μl loop or swab, three to five colonies of tested organisms were inoculated in a straight line from the edge of the disk to the edge of the plat up to four isolates can be tested on the same plate with one drug.
Identical plate precision was determined by analyzing six spots of the mixed standards solution (10 μL) on one plate, while different plate precision was tested by determining one spot of the mixed standards solution (10 μL) on six TLC plates, respectively.
We had tested various msh6 strains, but the reversion rate in msh6 strains is so low that one cannot obtain a reasonable number of revertants on one plate.
All on one plate.
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CEO of Professional Science Editing for Scientists @ prosciediting.com