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Since LiCl may have additional effects on cells beside GSK-3β inhibition, we also tested more specific reagents, GSK inhibitor VIII and XIII, which inhibit GSK-3β.
Because caffeine is a large spectrum PIKK inhibitor we tested more specific kinase inhibitors such as the specific ATM inhibitor (KU55933), wortmannin, and DNA-PK inhibitor (NU7026).
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More drug studies are indeed required, and future research needs to incorporate better diagnostic tools to test more specific and tailored therapies that account for underlying aetiologies and individual responsiveness.
When testing more specific hypotheses, other methods of classification (e.g., restricting the DI in space and time, template-matching, Mahalanobis distance) could be chosen.
Further research might also build on our findings to test more specific relationships between large-scale change implementation processes and intermediate and final outcomes.
It is, of course, possible that studies testing more specific hypotheses, for example along an elevational cline (as reported for Salix [ 11]), may uncover evidence for shifting sex ratios or for sexual dimorphism.
To confirm the results obtained by those screening tests more specific, fast and sensitive techniques could be used.
We also used a test more specific for balance and movement coordination.
Our topology also allows testing more specific evolutionary hypotheses, such as the position of the enigmatic mite family Pediculochelidae (Paralycus).
In conjunction with a compatible clinical presentation, SAT titres above 1 160 are considered diagnostic (19); however, in endemic areas, a titre of 1 320 as cutoff may make the test more specific.
Based on the findings of the present investigation, it would be valuable in future studies to state and test more specific hypotheses regarding changes in GM development in ASD, e.g., regarding the age at which the most severe changes in GM development occurs.
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