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To investigate whether the CXXC domain is needed for enzymatic activity or substrate recognition, we tested formation of the covalent complex with cytosine and transfer of the methyl group for GFP-Dnmt1 and GFP-Dnmt1ΔCXXC.
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They tested formations of the mineral that had grown atop paintings in 11 caves, assuming that whatever its age, the underlying paint had to be at least as old, and possibly much older.
To test formation of tightly packed domains and changes in membrane fluidity, we utilized a fluorescence technique that involves quenching of a membrane probe DPH by a quencher 2,2,6,6-tetramethylpiperdine-1-oxyl, TEMPO [21].
We first tested whether formation of DSS1-protein adducts could be regulated by ATP.
For non-viral gene delivery, we have developed and tested the formation of nanoparticles which consist of a stable aqueous dispersion of ultrafine ORMOSIL nanoparticles.
We also tested biofilm formation under anaerobic conditions (to better approximate conditions found in the human gastrointestinal tract[25]) on both PP and PS substrates.
We tested the formation of neurospheres in each group.
We next tested for formation of the ternary complex.
Finally, we tested the formation of DNA origami lattices on SUVs in suspension.
We tested chain formation, using either Ube2D3 (UbcH5c) or Ube2L3 (UbcH7) as E2 enzymes.
We then tested colony formation in soft agar as an indicator of cellular transformation.
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